Professor
Department of Biological Sciences
📍 3F4, Academic Block-1
✉️
 |
 |
 |
 |
Research Area
Immunology (Inflammation and Cell Death)
Research Interests
Inflammation is the manifestation of innate immune responses against the pathogen or tissue damage. Sterile inflammation or persistent chronic inflammation can cause several autoimmune diseases even cancer. Our lab is mainly interested in looking at how inflammatory pathways are modulated by the gram-negative enteric bacteria that cause gastroenteritis. Understanding of the pathogenesis pattern and working out the inflammatory immune signaling network are one of the main focus of our lab. As pathogenic bacteria bring an arsenal of protein factors to manipulate the host system, understanding the role of these factors in modulation of the host cellular responses is critical to the detailed understanding of the bacterial pathogenesis and eventual better designing of vaccine and therapy. Towards understanding the roles of gram-negative bacterial ligands in pathogenesis and modulation of innate/inflammatory responses, research of our lab currently focused on the following aims:
-Elucidating the molecular mechanism of innate immune responses/inflammation;
-Characterization of the immune signal network;
-Study of the effect of innate immune responses on the adaptive response;
-Study of the cell death pathways;
-Implications for the bacterial pathogenesis, host-pathogen interactions and immune responses.
2004 - 2009 : Post-doctoral research, Albert Einstein College of Medicine, Bronx, New York, USA
1999 - 2004 : National Institute of Cholera and Enteric Diseases, Kolkata, West Bengal (PhD awarded by Jadavpur University in 2005)
MSc (Botany) from University of Burdwan, West Bengal
Best-postdoctoral research award of Albert Einstein College of Medicine, New York (2008).
Juvenile Diabetes Research Fellowship, JDRF foundation (2007).
Young Scientist Award of the Indian Science Congress (2001).
Dwipjyoti Sarma, Vinica Dhar and Arunika Mukhopadhaya. Understanding the role of scavenger receptor CD36 in modulation of dendritic cell responses. Indian Journal of Inflammation Research 7(1):01–01, 2023.
Pratima Verma, Aakanksha Chauhan, Reena Thakur, Kusum Lata, Arpita Sharma, Kausik Chattopadhyay and Arunika Mukhopadhaya. Vibrio parahaemolyticus thermostable direct haemolysin induces non-classical programmed cell death despite caspase activation. Molecular Microbiology 120(6):845–873, 2023.
Vinica Dhar, Shraddha Gandhi, Sanica C Sakharwade, Amanpreet Chawla and Arunika Mukhopadhaya. Vibrio cholerae porin OmpU activates dendritic cells via TLR2 and the NLRP3 inflammasome. Infection and Immunity 91(2):e00332–22, 2023.
Shashi Prakash Yadav, Sanjeev Routh and Arunika Mukhopadhaya. Toll-Like Receptors (TLRs): Host immunity and bacterial ligand recognition.. , 2022.
Arpita Sharma, Shashi Prakash Yadav, Dwipjyoti Sarma and Arunika Mukhopadhaya. Chapter Two-Modulation of host cellular responses by gram-negative bacterial porins Author links open overlay panel. , 2022.
Deepinder Kaur, Pratima Verma, Mahendra Singh, Arpita Sharma, Kusum Lata, Arunika Mukhopadhaya and Kausik Chattopadhyay. Pore formation-independent cell death induced by a $\beta$-barrel pore-forming toxin. The FASEB Journal 36(10):e22557, 2022.
Arpita Sharma, Shashi Prakash Yadav, Dwipjyoti Sarma and Arunika Mukhopadhaya. Modulation of host cellular responses by gram-negative bacterial porins. Advances in Protein Chemistry and Structural Biology 128:35–77, 2022.
Mayank Joshi, Indu Verma, Aakanksha Gulati, Arunika Mukhopadhaya and Angshuman Roy Choudhury. New salts of levofloxacin with physiochemical, structural and biological insights.. , 2021.
Shilauni Dadwal. Murine-$\beta$-coronavirus infection differently induces" Interferon-induced protein with tetratricopeptide repeats 2 (Ifit2)" in Neuroglial cells. IISERM, 2021.
Manisha Kalsain. Association of Salmonella Typhimurium effector protein SteA with host cellular proteins and its role in cell death mechanisms. IISERM, 2021.
Deepinder Kaur and Arunika Mukhopadhaya. Understanding the Role of OmpV, an Outer Membrane Protein of Salmonella Typhimurium Towards Bacterial Pathogenesis and Host Immune Activation. IISERM, 2021.
Celina Meena and Arunika Mukhopadhaya. Characterization of cell death responses induced by salmonella enetrica typhimurium effector protein stea. IISERM, 2021.
Yogesh Saxena, Sanjeev Routh and Arunika Mukhopadhaya. Immunoporosis: role of innate immune cells in osteoporosis. Frontiers in immunology 12:687037, 2021.
Rohit Kapila, Mayank Kashyap, Aakanksha Gulati, Aaditya Narasimhan, Soumyadip Poddar, Arunika Mukhopadhaya and Nagaraj Guru Prasad. Evolution of sex-specific heat stress tolerance and larval Hsp70 expression in populations of Drosophila melanogaster adapted to larval crowding. Journal of Evolutionary Biology 34(9):1376–1385, 2021.
Deepinder Kaur, Shraddha Gandhi and Arunika Mukhopadhaya. Salmonella typhimurium adhesin OmpV activates host immunity to confer protection against systemic and gastrointestinal infection in mice. Infection and Immunity 89(8), 2021.
Arunika Mukhopadhaya and others. Role of Salmonella enterica Typhimurium secreted effector Protein, SteA in Host Cell Infection. IISERM, 2020.
Shubham Gajrani. Characterization of Salmonella effector protein SteA using MS-Proteomics analysis, Flow Cytometry and Western Blotting. IISERM, 2020.
Arunika Mukhopadhaya, Sinha Somdatta and others. Epidemiological Compartment Modelling of Malaria and Analyses of P. falciparum Prevalence Data in India. IISERM, 2020.
Deepinder Kaur and Arunika Mukhopadhaya. Outer membrane protein OmpV mediates Salmonella enterica serovar typhimurium adhesion to intestinal epithelial cells via fibronectin and $\alpha$1$\beta$1 integrin. Cellular Microbiology 22(5):e13172, 2020.
Aakanksha Gulati and Arunika Mukhopadhaya. Unravelling the Host Immunomodulation by Two Gram-negative Enteric Bacterial Ligands. IISERM, 2019.
Aakanksha Gulati, Rhythm Shukla and Arunika Mukhopadhaya. Salmonella effector SteA suppresses proinflammatory responses of the host by interfering with I$\kappa$B degradation. Frontiers in Immunology 10:493505, 2019.
GVR Prasad, Vinica Dhar and Arunika Mukhopadhaya. Vibrio cholerae OmpU mediates CD36-dependent reactive oxygen species generation triggering an additional pathway of MAPK activation in macrophages. The Journal of Immunology 202(8):2431–2450, 2019.
Aakanksha Gulati, Ranjai Kumar and Arunika Mukhopadhaya. Differential recognition of Vibrio parahaemolyticus OmpU by Toll-like receptors in monocytes and macrophages for the induction of proinflammatory responses. Infection and immunity 87(5):10–1128, 2019.
Aakanksha Gulati, Deepinder Kaur, GVR Krishna Prasad and Arunika Mukhopadhaya. PRR function of innate immune receptors in recognition of bacteria or bacterial ligands. Biochemical and biophysical roles of cell surface molecules, pages 255–280, 2018.
Arunika Mukhopadhaya. Nobel Prize in Physiology or Medicine–2018. Resonance 23:1359–1366, 2018.
Sumyra Sidiq, GVR Krishna Prasad, Arunika Mukhopadhaya and Santanu Kumar Pal. Poly (l-lysine)-coated liquid crystal droplets for cell-based sensing applications. The Journal of Physical Chemistry B 121(16):4247–4256, 2017.
Shelly Gupta. Study of Modulation of Host-cell Death by Vibrio cholerae Porin OmpU and the Underlying Mechanism. IISERM, 2016.
Shelly Gupta, GVR Krishna Prasad and Arunika Mukhopadhaya. OmpU induces caspase-independent programmed cell death. , 2015.
Sanica C Sakharwade and Arunika Mukhopadhaya. Vibrio cholerae porin OmpU induces LPS tolerance by attenuating TLR-mediated signaling. Molecular Immunology 68(2):312–324, 2015.
Shelly Gupta, GVR Krishna Prasad and Arunika Mukhopadhaya. Vibrio cholerae porin OmpU induces caspase-independent programmed cell death upon translocation to the host cell mitochondria. Journal of Biological Chemistry 290(52):31051–31068, 2015.
Sanica C Sakharwade, GVR Krishna Prasad and Arunika Mukhopadhaya. Immuno-modulatory role of porins: host immune responses, signaling mechanisms and vaccine potential. In Biochemical Roles of Eukaryotic Cell Surface Macromolecules. 2015, 79–108.
Junaid Khan, Praveen K Sharma and Arunika Mukhopadhaya. Vibrio cholerae porin OmpU mediates M1-polarization of macrophages/monocytes via TLR1/TLR2 activation. Immunobiology 220(11):1199–1209, 2015.
Barkha Khilwani, Arunika Mukhopadhaya and Kausik Chattopadhyay. Transmembrane oligomeric form of Vibrio cholerae cytolysin triggers TLR2/TLR6–dependent proinflammatory responses in monocytes and macrophages. Biochemical Journal 466(1):147–161, 2015.
Sanica C Sakharwade, Praveen K Sharma and Arunika Mukhopadhaya. Vibrio cholerae porin OmpU induces pro-inflammatory responses, but down-regulates LPS-mediated effects in RAW 264.7, THP-1 and human PBMCs. PLoS One 8(9):e76583, 2013.
Junaid Khan, Shelly Gupta, Kausik Chattopadhyay and Arunika Mukhopadhaya. Refolding and functional assembly of the Vibrio cholerae porin OmpU recombinantly expressed in the cytoplasm of Escherichia coli. Protein Expression and Purification 85(2):204–210, 2012.
Arunika Mukhopadhaya, Tadashi Hanafusa, Irene Jarchum, Yi-Guang Chen, Yoshiko Iwai, David V Serreze, Ralph M Steinman, Kristin V Tarbell and Teresa P DiLorenzo. Selective delivery of $\beta$ cell antigen to dendritic cells in vivo leads to deletion and tolerance of autoreactive CD8+ T cells in NOD mice. Proceedings of the National Academy of Sciences 105(17):6374–6379, 2008.
Kausik Chattopadhyay, Udupi A Ramagopal, Arunika Mukhopadhaya, Vladimir N Malashkevich, Teresa P DiLorenzo, Michael Brenowitz, Stanley G Nathenson and Steven C Almo. Assembly and structural properties of glucocorticoid-induced TNF receptor ligand: Implications for function. Proceedings of the National Academy of Sciences 104(49):19452–19457, 2007.
Arunika Mukhopadhaya, Joseph Mendecki, Xinyuan Dong, Laibin Liu, Shalom Kalnicki, Madhur Garg, Alan Alfieri and Chandan Guha. Localized hyperthermia combined with intratumoral dendritic cells induces systemic antitumor immunity. Cancer research 67(16):7798–7806, 2007.
Erhu Cao, Xingxing Zang, Udupi A Ramagopal, Arunika Mukhopadhaya, Alexander Fedorov, Elena Fedorov, Wendy D Zencheck, Jeffrey W Lary, James L Cole, Haiteng Deng and others. T cell immunoglobulin mucin-3 crystal structure reveals a galectin-9-independent ligand-binding surface. Immunity 26(3):311–321, 2007.
A Mukhopadhaya, D Mahalanabis and MK Chakrabarti. Role of Shigella flexneri 2a 34 kDa outer membrane protein in induction of protective immune response. Vaccine 24(33-34):6028–6036, 2006.
Arunika Mukhopadhaya, Joseph Mendecki, Kosho Yamanouchi, Laibin Liu, Shalin Shah, Indranil Basu, Alan Alfieri, Madhur Garg, Shalom Kalnicki and Chandan Guha. Localized hyperthermia combined with intratumoral dendritic cell injection induces systemic antitumor immunity. Cancer Research 65(9_Supplement):171–171, 2005.
A Mukhopadhaya, D Mahalanabis, J Khanam and MK Chakrabarti. Protective efficacy of oral immunization with heat-killed Shigella flexneri 2a in animal model: study of cross protection, immune response and antigenic recognition. Vaccine 21(21-22):3043–3050, 2003.