Associate Professor
Department of Biological Sciences
📍 3F10, AB1
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Research Area
Regeneration Biology
Research Interests
Our research interests are to understand the molecular mechanisms underlying retina and limb regeneration using zebrafish and axolotl models. Though the cellular and molecular basis of this remarkable phenomenon remains largely unknown, recent studies have suggested the detrimental role of various genetic and epigenetic factors contribute to tissue regeneration. However, the wealth of knowledge is not sufficient to take this to the next stage, where humans can get benefited out of this. In this scenario, we are working on various signaling pathways to assess their impact on fish and axolotl regeneration. We are also working on how diabetes or hyperglycemia impedes tissue regeneration and the molecular mechanisms causing it.
Since 2018 : Associate professor, IISER Mohali
2012 - 2018 : Assistant professor, IISER Mohali
2005 - 2007 : Postdoctoral Fellow, CCMB, Hyderabad, India
2007 - 2012 : Postdoctoral Fellow, University of Michigan Ann Arbor, USA
1999 - 2005 : Ph.D. Scholar, CCMB, Hyderabad, India/ Degree awarded by JNU, New Delhi
1997 - 1999 : M.Sc. Marine Biology, Microbiology, Biochemistry/ CUSAT Cochin, Kerala
1993 - 1997 : B.Sc. Zoology, Chemistry, Botany (Hons), St. Michaels College, University of Kerala
Wellcome Trust-DBT India Alliance Intermediate fellowship (2013-2017). A five-year Grant awarded by the Department of Biotechnology (DBT), India and the Wellcome Trust, UK. INR-324 lakhs
Postdoctoral fellowship at University of Michigan (2007-2008). Part of NIH sponsored project on retina regeneration awarded to Prof. Daniel Goldman. Grant # NIH NEI RO1 EY018132
Postdoctoral research grant from National Institute of Health (NIH), Bethesda, MD, USA. NIH, NICHD; Grant# T32HD00750MBNI, Centre for Organogenesis-University of Michigan, USA. Awarded to Rajesh Ramachandran during academic year 2008-2009.
Prof. N.J. Chinoy Award for the best paper presentation in the XXIII SRBCE (Society for Reproductive Biology and Comparative Endocrinology) symposium held at Visva Bharati University, Santiniketan, Kolkata, India during February 7-9, 200
Post Doctoral Fellowship, Department of Biotechnology (DBT), India (2005-2007)
Senior Research Fellowship, Indian Council of Agriculture Research (ICAR), India (2004-2005)
Senior Research Fellowship, Council of Scientific and Industrial Research(CSIR), India (2003-2004)
Senior Research Fellowship, University Grants Commission (UGC-NET), India (2001-2003)
Junior Research Fellowship, University Grants Commission (UGC-NET), India (1999-2001)
University Rank 1st in M.Sc. Marine Biology, Cochin University of Science and Technology (CUSAT), Kerala, India (1999).
Shivangi Gupta, Poonam Sharma, Mansi Chaudhary, Sharanya Premraj, Simran Kaur, Vijithkumar Vijayan, Manas Geeta Arun, Nagaraj Guru Prasad and Rajesh Ramachandran. Pten associates with important gene regulatory network to fine-tune Müller glia-mediated zebrafish retina regeneration. Glia 71(2):259–283, 2023.
Rajesh Ramachandran. Aquaculture Productivity Enhancement Through Advanced Technologies. , 2022.
Kshitish C Majumdar and Rajesh Ramachandran. Aquaculture Productivity Enhancement Through Advanced Technologies. In Advances in Fisheries Biotechnology. Springer, 2022, pages 1–28.
Poonam Sharma and Rajesh Ramachandran. Retina regeneration: lessons from vertebrates. Oxford Open Neuroscience 1:kvac012, 2022.
Bindia Chawla, Anisha Pahuja and Rajesh Ramachandran. Exome sequencing identifies procollagen-lysine 2-oxoglutarate 5-dioxygenase 2 mutations in primary congenital and juvenile glaucoma. , 2021.
Pallavi Joshi and Rajesh Ramachandran. Understanding the Role of the Hippo-YAP Signaling Pathway in the Process of Retina Regeneration in Zebrafish. IISERM, 2021.
Rajesh Ramachandran and others. Study of factors involved in Chromatin remodeling during Zebrafish retina regeneration. IISERM, 2021.
Rajesh Ramachandran and others. Understanding the role of Matrix metalloprotease 14 (Mmp-14) and Furin during retina regeneration in zebrafish. IISERM, 2021.
Shivangi Gupta, Poonam Sharma, Mansi Chaudhary, Sharanya Premraj, Simran Kaur, V Vijithkumar and Rajesh Ramachandran. Temporal regulation of Pten is essential for retina regeneration in zebrafish. bioRxiv, pages 2021–07, 2021.
B Chawla, Rajesh Ramachandran and KS Sandhu. CTCF-Mediated Genome Architecture Regulates the Dosage of Mitotically Stable Mono-allelic Expression of Autosomal Genes. , 2020.
Rajesh Ramachandran and others. Studying Retinal Regeneration in Type 2 Diabetes Mellitus Zebrafish Model, Developed Through Overfeeding. IISERM, 2020.
Keerthivasan Raanin Chandradoss, Bindia Chawla, Shivnarayan Dhuppar, Rakhee Nayak, Rajesh Ramachandran, Sreenivasulu Kurukuti, Aprotim Mazumder and Kuljeet Singh Sandhu. CTCF-mediated genome architecture regulates the dosage of mitotically stable mono-allelic expression of autosomal genes. Cell Reports 33(4), 2020.
Rajesh Ramachandran. CRISPR/Cas9 System: 2020 Nobel Prize in Chemistry. Resonance 25(12):1669–1680, 2020.
Poonam Sharma, Shivangi Gupta, Mansi Chaudhary, Soumitra Mitra, Bindia Chawla, Mohammad Anwar Khursheed, Navnoor Kaur Saran and Rajesh Ramachandran. Biphasic role of Tgf-$\beta$ signaling during Müller glia reprogramming and retinal regeneration in zebrafish. IScience 23(2), 2020.
Komal Meena. Understanding The Role of zebs and cadherins in Zebrafish Retina Regeneration. IISER M, 2019.
Poonam Sharma and Rajesh Ramachandran. Retina injury and retina tissue preparation to study regeneration in zebrafish. Bio-protocol 9(24):e3466–e3466, 2019.
Shambhu Yadav, Bindia Chawla, Mohammad Anwar Khursheed, Rajesh Ramachandran and Anand Kumar Bachhawat. The glutathione degrading enzyme, Chac1, is required for calcium signaling in developing zebrafish: redox as an upstream activator of calcium. Biochemical Journal 476(13):1857–1873, 2019.
Poonam Sharma, Shivangi Gupta, Mansi Chaudhary, Soumitra Mitra, Bindia Chawla, Mohammad Anwar Khursheed and Rajesh Ramachandran. Oct4 mediates Müller glia reprogramming and cell cycle exit during retina regeneration in zebrafish. Life science alliance 2(5), 2019.
Soumitra Mitra, Poonam Sharma, Simran Kaur, Mohammad Anwar Khursheed, Shivangi Gupta, Mansi Chaudhary, Akshai J Kurup and Rajesh Ramachandran. Dual regulation of lin28a by Myc is necessary during zebrafish retina regeneration. Journal of Cell Biology 218(2):489–507, 2019.
Soumitra Mitra, Poonam Sharma, Simran Kaur, Mohammad Anwar Khursheed, Shivangi Gupta, Riya Ahuja, Akshai J Kurup, Mansi Chaudhary and Rajesh Ramachandran. Histone deacetylase-mediated Müller glia reprogramming through Her4. 1-Lin28a axis is essential for retina regeneration in zebrafish. IScience 7:68–84, 2018.
Simran Kaur, Shivangi Gupta, Mansi Chaudhary, Mohammad Anwar Khursheed, Soumitra Mitra, Akshai Janardhana Kurup and Rajesh Ramachandran. let-7 MicroRNA-mediated regulation of Shh signaling and the gene regulatory network is essential for retina regeneration. Cell reports 23(5):1409–1423, 2018.
Priyanka Sudheendra Jamadagni. Investigating the Role and Regulation of Ezh2 in Zebrafish Retina Regeneration. IISER-M, 2016.
Rajesh Ramachandran. Leptin and IL-6 Family Cytokines Synergize to Stimulate Müller Glia Reprogramming and Retina Regeneration. , 2014.
Xiao-Feng Zhao, Jin Wan, Curtis Powell, Rajesh Ramachandran, Martin G Myers and Daniel Goldman. Leptin and IL-6 family cytokines synergize to stimulate Müller glia reprogramming and retina regeneration. Cell reports 9(1):272–284, 2014.
Rajesh Ramachandran, Aaron Reifler, Jin Wan and Daniel Goldman. Application of Cre-loxP recombination for lineage tracing of adult zebrafish retinal stem cells. Retinal Development: Methods and Protocols, pages 129–140, 2012.
Jin Wan, Rajesh Ramachandran and Daniel Goldman. HB-EGF is necessary and sufficient for Müller glia dedifferentiation and retina regeneration. Developmental cell 22(2):334–347, 2012.
Rajesh Ramachandran, Xiao-Feng Zhao and Daniel Goldman. Insm1a-mediated gene repression is essential for the formation and differentiation of Müller glia-derived progenitors in the injured retina. Nature cell biology 14(10):1013–1023, 2012.
Rajesh Ramachandran, Xiao-Feng Zhao and Daniel Goldman. Ascl1a/Dkk/$\beta$-catenin signaling pathway is necessary and glycogen synthase kinase-3$\beta$ inhibition is sufficient for zebrafish retina regeneration. Proceedings of the National Academy of Sciences 108(38):15858–15863, 2011.
Rajesh Ramachandran, Aaron Reifler, Jack M Parent and Daniel Goldman. Conditional gene expression and lineage tracing of tuba1a expressing cells during zebrafish development and retina regeneration. Journal of Comparative Neurology 518(20):4196–4212, 2010.
Rajesh Ramachandran, Blake V Fausett and Daniel Goldman. Ascl1a regulates Müller glia dedifferentiation and retinal regeneration through a Lin-28-dependent, let-7 microRNA signalling pathway. Nature cell biology 12(11):1101–1107, 2010.
Steven T Suhr, Rajesh Ramachandran, Cynthia L Fuller, Matthew B Veldman, Christine A Byrd and Daniel Goldman. Highly-restricted, cell-specific expression of the simian CMV-IE promoter in transgenic zebrafish with age and after heat shock. Gene Expression Patterns 9(1):54–64, 2009.
R Rajesh and KC Majumdar. The growth hormone-encoding gene isolated and characterized from Labeo rohita Hamilton is expressed in CHO cells under the control of constitutive promoters in ‘autotransgene’constructs. Fish physiology and biochemistry 34:413–436, 2008.
R Rajesh and KC Majumdar. A comparative account of the structure of the growth hormone encoding gene and genetic interrelationship in six species of the genus Labeo. Fish Physiology and Biochemistry 33:311–333, 2007.
R Rajesh and KC Majumdar. Transgene integration-an analysis in autotransgenic Labeo rohita Hamilton (Pisces: Cyprinidae). Fish physiology and biochemistry 31:281–287, 2005.