Associate Professor
Department of Chemical Sciences
📍 1F12 / AB1
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Research Area
Mechanobiology
Research Interests
We are an interdisciplinary research group on Chemical-Biology who love to experiment with one molecule, one live-cell at a time, and extend to whole tissues. We believe that this simplistic philosophy will help us in understanding some of the exciting phenomena in life-sciences. By blending our knowledge of physical Sciences, biological Sciences and engineering, we aim to understand the most well-developed living-sensor, 'hearing' and aspire to establish diagnostic tools to understand the health of our body by listening to our ear. We utilize both experimental approaches using Atomic Force Microscope, single-molecule Forster Resonance Energy Transfer (FRET) using TIRF Microscope, Fluorescence Imaging, Live-cell imaging, Time-Resolved Emission Spectroscopy (TRES), Near-Field Scanning Optical Microscopy (NSOM) etc. as well as computational approaches like Molecular Dynamics and Steered Molecular Dynamics. Please visit our Research page and facilities to learn more about us.
Since 2023 : Member of Fluorescence Society of India
2009 - 2013 : Postdoctoral Fellow, Iowa State University, USA
2003 - 2009 : PhD, Indian Institute of Science, Bengaluru, India
2000 - 2002 : MSc in Chemistry, Burdwan University, WB, India
1997 - 2000 : BSc in Chemistry, Burdwan University, WB, India
Sabyasachi Rakshit Sharmistha Sinha S M Rose Silky Bedi. Substrate-Induced Phase Transition within Liquid Condensates Reverses Catalytic Activity of Nanoparticles. Nanoscale, 2024. Nisha Arora, Jagadish P Hazra, Sandip Roy, Gaurav K Bhati, Sarika Gupta, KP Yogendran, Abhishek Chaudhuri, Amin Sagar and Sabyasachi Rakshit. Emergence of slip-ideal-slip behavior in tip-links serve as force filters of sound in hearing. Nature Communications 15(1):1595, 2024. Veerpal Kaur, Surbhi Garg and Sabyasachi Rakshit. Polyprotein synthesis: a journey from the traditional pre-translational method to modern post-translational approaches for single-molecule force spectroscopy. Chemical Communications 59(46):6946–6955, 2023. Veerpal Kaur, Sanat K Ghosh, Tripta Bhatia and Sabyasachi Rakshit. Redefining the structure of tip links in hair cells. Biochemistry 62(15):2244–2251, 2023. Cheerneni S Srinivas, Gayathri S Singaraju, Veerpal Kaur, Sayan Das, Sanat K Ghosh, Amin Sagar, Anuj Kumar, Tripta Bhatia and Sabyasachi Rakshit. Transient interactions drive the lateral clustering of cadherin-23 on membrane. Communications Biology 6(1):293, 2023. Silky Bedi, Gaurav Kumar, SM Rose, Sabyasachi Rakshit and Sharmistha Sinha. Barrier-free liquid condensates of nanocatalysts as effective concentrators of catalysis. Chemical Communications 58(62):8634–8637, 2022. Veerpal Kaur, Surbhi Garg and Sabyasachi Rakshit. Instantaneous splicing and excision of inteins to synthesize polyproteins on a substrate with tunable linkers. Soft Matter 18(3):602–608, 2022. Nisha Arora, Jagadish Prasad Hazra and Sabyasachi Rakshit. Anisotropy in mechanical unfolding of protein upon partner-assisted pulling and handle- assisted pulling. Communications Biology 4(1):925, 2021. Tanuja Joshi, Surbhi Garg, Alejandro Estaña, Juan Cortés, Pau Bernadó, Sayan Das, Anjana R Kammath, Amin Sagar and Sabyasachi Rakshit. Interdomain linkers tailor the stability of immunoglobulin repeats in polyproteins. Biochemical and Biophysical Research Communications 550:43–48, 2021. Sheetal Rani, Basundhara Dasgupta, Gaurav Kumar Bhati, Kalpana Tomar, Sabyasachi Rakshit and Subhabrata Maiti. Superior proton-transfer catalytic promiscuity of cytochrome c in self-organized media. ChemBioChem 22(7):1285–1291, 2021. Gayathri Singaraju Rahul Dani Naimat Bari Athi Naganathan* Sabyasachi Rakshit* S K N Surbhi Garg Amin Sagar. Weakening of Interaction Networks with Aging in Tip-Link Proteins Induces Hearing Loss. Biochemical J 478(1):121–134, 2021. Gayathri S Singaraju, Amin Sagar, Anuj Kumar, Jesse S Samuel, Jagadish P Hazra, Malay K Sannigrahi, Ragothaman M Yennamalli, Ashish and Sabyasachi Rakshit. Structural basis of the strong cell-cell junction formed by cadherin-23. The FEBS journal 287(11):2328–2347, 2020. Malay Kumar Sannigrahi Sabyasachi Rakshit* Sharmistha Sinha* Ankush Garg Jagadish Prasad Hazra. Variable Mutations at the p53-R273 Oncogenic Hotspot Position Leads to Altered Properties. BioPhys. J., 2019. Jagadish P Hazra, Amin Sagar, Nisha Arora, Debadutta Deb, Simerpreet Kaur and Sabyasachi Rakshit. Broken force dispersal network in tip-links by the mutations at the Ca2+-binding residues induces hearing-loss. Biochemical Journal 476(16):2411–2425, 2019. Malay K Sannigrahi, Cheerneni S Srinivas, Nilesh Deokate and Sabyasachi Rakshit. The strong propensity of Cadherin-23 for aggregation inhibits cell migration. Molecular oncology 13(5):1092–1109, 2019. GS Singaraju, S Rakshit and F Ashish. Molecular structure of Cadherin-23 mediated strong cell-cell adhesion.. In MOLECULAR BIOLOGY OF THE CELL 29(26). 2018. Malay Kumar Sannigrahi, Sai Srinivas and Sabyasachi Rakshit. The prospects of cadherin-23 as a mediator of homophilic cell-cell adhesion. Biochemical and Biophysical Roles of Cell Surface Molecules, pages 99–105, 2018. Jagadish P Hazra, Nisha Arora, Amin Sagar, Shwetha Srinivasan, Abhishek Chaudhuri and Sabyasachi Rakshit. Force-activated catalytic pathway accelerates bacterial adhesion against flow. Biochemical Journal 475(16):2611–2620, 2018. Surbhi Garg, Gayathri Sindhuri Singaraju, Sunanda Yengkhom and Sabyasachi Rakshit. Tailored polyproteins using sequential staple and cut. Bioconjugate chemistry 29(5):1714–1719, 2018. Shwetha Srinivasan, Jagadish P Hazra, Gayathri S Singaraju, Debadutta Deb and Sabyasachi Rakshit. ESCORTing proteins directly from whole cell-lysate for single-molecule studies. Analytical biochemistry 535:35–42, 2017. Kristine Manibog, Hui Li, Sabyasachi Rakshit and Sanjeevi Sivasankar. Resolving the molecular mechanism of cadherin catch bond formation. Nature communications 5(1):3941, 2014. Molly Lowndes, Sabyasachi Rakshit, Omer Shafraz, Nicolas Borghi, Robert M Harmon, Kathleen J Green, Sanjeevi Sivasankar and James W Nelson. Different roles of cadherins in the assembly and structural integrity of the desmosome complex. Journal of cell science 127(10):2339–2350, 2014. Sabyasachi Rakshit and Sanjeevi Sivasankar. Biomechanics of cell adhesion: how force regulates the lifetime of adhesive bonds at the single molecule level. Physical Chemistry Chemical Physics 16(6):2211–2223, 2014. Sabyasachi Rakshit, Yunxiang Zhang, Kristine Manibog, Omer Shafraz and Sanjeevi Sivasankar. Ideal, catch, and slip bonds in cadherin adhesion. Proceedings of the National Academy of Sciences 109(46):18815–18820, 2012. S Sivasankar, S Rakshit, Y Zhang, K Manibog and O Shafraz. Resolving cadherin interactions at the single molecule level. In JOURNAL OF VASCULAR RESEARCH 48. 2011, 198–198. Sabyasachi Rakshit and Sanjeevi Sivasankar. Cross-linking of a charged polysaccharide using polyions as electrostatic staples. Soft Matter 7(6):2348–2351, 2011. Sabyasachi Rakshit and Sukumaran Vasudevan. Spectrally resolved resonance energy transfer from ZnO: MgO nanocrystals. The Journal of Physical Chemistry C 113(37):16424–16431, 2009. Sabyasachi Rakshit and Sukumaran Vasudevan. Resonance energy transfer from $\beta$-cyclodextrin-capped ZnO: MgO nanocrystals to included Nile Red guest molecules in aqueous media. ACS nano 2(7):1473–1479, 2008. Sabyasachi Rakshit and Sukumaran Vasudevan. Trap-state dynamics in visible-light-emitting ZnO: MgO nanocrystals. The Journal of Physical Chemistry C 112(12):4531–4537, 2008.